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Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus

Paper ID Volume ID Publish Year Pages File Format Full-Text
17015 42632 2014 4 PDF Available
Title
Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus
Abstract

•Metabolic engineering of K. marxianus for 2-phenylethanol production was attempted.•Evolved strain producing high level of 2-PE was isolated.•Bacterial aroGfbr was overexpressed in K. marxianus using an expression vector.•1.3 g/L of 2-PE was produced from glucose without addition of phenylalanine.

2-Phenylethanol (2-PE) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-PE is produced in a few yeast strains by Ehrlich pathway. In this study, Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose. About 1.0 g/L of 2-PE was produced by overexpressing phenylpyruvate decarboxylase (ARO10) and alcohol dehydrogenase (ADH2) genes of Saccharomyces cerevisiae. A similar level of 2-PE was also produced from evolved K. marxianus, which was resistant to the phenylalanine analog, p-fluorophenylalanine. aroGfbr from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DHAP) synthase was overexpressed in the evolved K. marxianus. Finally, 1.3 g/L of 2-PE was produced from 20 g/L glucose without addition of phenylalanine in the medium.

Keywords
2-Phenylethanol; DHAP synthase; Kluyveromyces marxianus; Ehrlich pathway
First Page Preview
Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 61–62, July–August 2014, Pages 44–47
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering