fulltext.study @t Gmail

Proteomics analysis of Bacillus licheniformis in response to oligosaccharides elicitors

Paper ID Volume ID Publish Year Pages File Format Full-Text
17018 42632 2014 6 PDF Available
Title
Proteomics analysis of Bacillus licheniformis in response to oligosaccharides elicitors
Abstract

•Changes by oligosaccharide elicitors in the proteomic profile of B. licheniformis.•Identification of nineteen differentially expressed proteins.•Proteins involved in carbon metabolism, amino acid biosynthesis, energy generation.•Proteins involved in oxidative and general stress response.

The role of oligosaccharides as biotic elicitors has been recognised in the enhanced production of antibiotics from fungal and bacterial cultures. The yield of bacitracin A in cultures of Bacillus licheniformis was increased after supplementation with oligoguluronate (OG), and mannan oligosaccharides (MO) and its mechanism at transcription level been established already. However, the elicitation mechanism at post transcriptional level has not been reported so far. In this paper we investigate changes in proteomics of B. licheniformis in presence of the oligosaccharide elicitors OG and MO. Differentially expressed proteins were examined using 2D-PAGE stained with colloidal Coomassie and were further identified by LC–MS/MS. We identified 19 differentially expressed proteins including those involved in carbon metabolism, energy generation, amino acid biosynthesis, oxidative and general stress response. The novel findings of this work, together with previous reports, contribute to the unravelling of the overall mechanism of elicitation in B. licheniformis cultures and reliability of the use of these elicitors for potential industrial application.

Keywords
Bacillus licheniformis; Biotic elicitation; Proteomics; Adaptation; Mechanism of action
First Page Preview
Proteomics analysis of Bacillus licheniformis in response to oligosaccharides elicitors
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 61–62, July–August 2014, Pages 61–66
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us