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A new role for penicillin acylases: Degradation of acyl homoserine lactone quorum sensing signals by Kluyvera citrophila penicillin G acylase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17022 42633 2014 7 PDF Available
Title
A new role for penicillin acylases: Degradation of acyl homoserine lactone quorum sensing signals by Kluyvera citrophila penicillin G acylase
Abstract

•First report on AHL acylase activity of a well-known penicillin G acylase.•Kinetics of AHL degradation reaction catalyzed by KcPGA.•Molecular modeling and docking studies support the findings.

Use of penicillin acylases for the production of semi-synthetic penicillins is well-known. Escherichia coli penicillin G acylase (EcPGA) has been extensively used for this purpose; however, Kluyvera citrophila penicillin G acylase (KcPGA) is assumed to be a better substitute, owing to its increased resilience to extreme pH conditions and ease of immobilization. In the present article we report a new dimension for the amidase activity of KcPGA by demonstrating its ability to cleave bacterial quorum sensing signal molecules, acyl homoserine lactones (AHL) with acyl chain length of 6–8 with or without oxo-substitution at third carbon position. Initial evidence of AHL degrading capability of KcPGA was obtained using CV026 based bioassay method. Kinetic studies performed at pH 8.0 and 50 °C revealed 3-oxo-C6 HSL to be the best substrate for the enzyme with Vmax and Km values of 21.37 + 0.85 mM/h/mg of protein and 0.1 + 0.01 mM, respectively. C6 HSL was found to be the second best substrate with Vmax and Km value of 10.06 + 0.27 mM/h/mg of protein and 0.28 + 0.02 mM, respectively. Molecular modeling and docking studies performed on the active site of the enzyme support these findings by showing the fitting of AHLs perfectly within the hydrophobic pocket of the enzyme active site.

Keywords
KcPGA; Quorum quenching; Acyl homoserine lactones; CV026
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A new role for penicillin acylases: Degradation of acyl homoserine lactone quorum sensing signals by Kluyvera citrophila penicillin G acylase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 56, 5 March 2014, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us