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Effect of surface charge alteration on stability of l-asparaginase II from Escherichia sp.

Paper ID Volume ID Publish Year Pages File Format Full-Text
17024 42633 2014 5 PDF Available
Title
Effect of surface charge alteration on stability of l-asparaginase II from Escherichia sp.
Abstract

•E. colil-asparaginases have great significance in the treatment of leukemia.•The effect of surface charge on stability of l-asparaginase II was studied by site directed mutagenesis.•We mutated two positively charged residues on the surface loops.•Two mutants with neutral charge retained greater tolerance and stability.

Escherichia colil-asparaginases have great significance in the treatment of leukemia. Consequently, there is considerable interest in engineering this enzyme for improving its stability. In this work, the effect of surface charge on the stability of the enzyme l-asparaginase II was studied by site-directed mutagenesis of the cloned ansB gene from Escherichia sp. Replacement of two positively charged residues (K139 and K207) on the surface loops with neutral and reverse charges resulted in altered thermo stability in designed variants. Neutral charge substitutions (K139A and K207A) retained greater tolerance and stability followed by negative charge substitutions (K139D and K207D) compared to control mutant K139R and wild enzyme. From the results, it was concluded that the optimization of surface charge contributed much to the thermal properties of proteins without affecting the structure.

Keywords
l-Asparaginase; Site-directed mutagenesis; Surface charge alteration; Thermal tolerance
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Effect of surface charge alteration on stability of l-asparaginase II from Escherichia sp.
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 56, 5 March 2014, Pages 15–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us