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Effect of sub- and supercritical CO2 treatment on the properties of Pseudomonas cepacia lipase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17038 42635 2013 8 PDF Available
Title
Effect of sub- and supercritical CO2 treatment on the properties of Pseudomonas cepacia lipase
Abstract

•Most compressed CO2 treatments significantly enhanced PCL esterification activity.•High pressure treatment facilitated PCL's esterification reactions.•The secondary and tertiary structure changes were responsible for activity variations.•The stability of PCL significantly decreased in esterification after supercritical treatment.

In this work, we have investigated the influences of sub- and supercritical CO2 treatment on the properties of Pseudomonas cepacia lipase (PCL), including its esterification and transesterification activities, structural changes and stability. Results demonstrated that exposure time to subcritical CO2 treatment had a negative effect on PCL transesterification activity whereas exposure time to supercritical CO2 treatment had a positive effect. But generally, most compressed treatments significantly enhanced PCL esterification activity. Conformational analysis by FT-IR and fluorescence emission spectra revealed that enhanced activities after supercritical CO2 treatment were correlated with the secondary and tertiary structural changes of PCL. Secondary structure changes also appeared to be responsible for enhancement of PCL activities by subcritical CO2 treatment. Compared to native PCL, treated PCL's esterification activity significantly decreased in hydrophilic organic media, while transesterification activity significantly increased in tert-amyl alcohol and acetone. After supercritical treatment, the thermal stability of PCL significantly decreased in esterification reactions, however, there was no significant difference in transesterification reactions.

Keywords
Compressed carbon dioxide; Pseudomonas cepacia lipase (PCL); Fourier transform-infrared spectrometry (FT-IR); Fluorescence spectra; Organic solvent tolerance; Thermal stability
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Effect of sub- and supercritical CO2 treatment on the properties of Pseudomonas cepacia lipase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 2, 10 July 2013, Pages 110–117
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us