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Kinetic studies and model development for the formation of galacto-oligosaccharides from lactose using synthesized thermo-responsive bioconjugate

Paper ID Volume ID Publish Year Pages File Format Full-Text
17048 42636 2015 8 PDF Available
Title
Kinetic studies and model development for the formation of galacto-oligosaccharides from lactose using synthesized thermo-responsive bioconjugate
Abstract

•Galacto-oligosaccharides formed using synthesized thermo-responsive bioconjugate.•Poly-N-isopropylacrylamide-β-galactosidase was used in synthesizing bioconjugate.•The influences of operating parameters on the formation of GOS were investigated.•Thermo-responsive bioconjugate fetches a maximum of 35% GOS yield.•A five-step, nine-parameter kinetic model developed for the prediction of GOS yield.

In an earlier study by us [47], thermo-responsive bioconjugate (poly-N-isopropylacrylamide-β-galactosidase) was synthesized and characterized. This study utilizes the prowess of such smart bioconjugate for the enzymatic synthesis of galacto-oligosaccharides (GOS) from lactose at various initial lactose concentrations (ILC), enzyme concentrations, and temperatures, while maintaining a constant pH of 6. A maximum GOS yield of 35% (on dry basis) was observed at 100 g/L ILC and 0.275 mg/mL (0.055 U/mL) conjugated protein. The GOS yield remained approximately the same for 50 and 100 g/L ILC, beyond which, it decreased. As the enzyme concentration increased, the equilibrium formation of GOS increased and eventually attained a plateau when the concentration of conjugated protein exceeded 0.275 mg/mL (0.055 U/mL). GOS yield increased on raising the temperature from 30 to 40 °C, and declined thereafter. The apparent kinetic parameters were estimated from a five-step, nine-parameter kinetic model, which was then simulated using the COPASI package. The simulated results demonstrated an excellent match with the experimental data.

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Keywords
Enzyme immobilization; Galacto-oligosaccharide; Kinetic model; Lactose; Poly-N-isopropyl acrylamide; Responsive bioconjugate
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Kinetic studies and model development for the formation of galacto-oligosaccharides from lactose using synthesized thermo-responsive bioconjugate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 70, March 2015, Pages 42–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us