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Biosensing methods for xanthine determination: A review

Paper ID Volume ID Publish Year Pages File Format Full-Text
17054 42637 2014 8 PDF Available
Title
Biosensing methods for xanthine determination: A review
Abstract

•Review methods available for xanthine analysis with special emphasis on biosensors.•Xanthine biosensors work optimally within 4–150 s, between pH 5.0 and 8.4 and in range of 1–1000 μM.•Xanthine biosensors had detection limit in range 0.1–20 μM.•Biosensor measured xanthine level in fish meat.

Xanthine (3,7-dihydro-purine-2,6-dione) is generated from guanine by guanine deaminase and hypoxanthine by xanthine oxidase (XOD). The determination of xanthine in meat indicates its freshness, while its level in serum/urine provides valuable information about diagnosis and medical management of certain metabolic disorders such as xanthinuria, hyperurecemia, gout and renal failure. Although chromatographic methods such a HPLC, capillary electrophoresis and mass spectrometry are available for quantification of xanthine in biological materials, these suffer from certain limitations such as complexity, time consuming sample preparation and requirement of expensive apparatus and trained persons to operate. Immobilized XOD based biosensors have emerged as simple, rapid, sensitive and economic tools for determination of xanthine in food industries and clinical diagnosis. This review article describes the various immobilization methods of XOD and different matrices used for construction of xanthine biosensors, their classification, analytical performance and applications along with their merits and demerits. The future perspectives for improvement of present xanthine biosensors are also discussed.

Keywords
Xanthine; Xanthine oxidase; Xanthine biosensor; Immobilization; Nanomaterials; Meat; Serum; Urine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 57, 10 April 2014, Pages 55–62
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us