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A novel horseradish peroxidase biosensor towards the detection of dopamine: A voltammetric study

Paper ID Volume ID Publish Year Pages File Format Full-Text
17056 42637 2014 8 PDF Available
Title
A novel horseradish peroxidase biosensor towards the detection of dopamine: A voltammetric study
Abstract

•A novel reagent less biosensor for the determination of DA in clinical samples.•Biosensor showed high sensitivity, selectivity and fast response.•Low Kmapp of the biosensor showed a good affinity of HRP towards DA.

A polymerized film of glycine (Gly) was prepared on the surface of carbon paste electrode (CPE) through the cyclic voltammetry (CV) technique. A novel biosensor for the determination of dopamine (DA) has been constructed based on horseradish peroxidase (HRP) and multiwalled carbon nanotubes (MWCNTs) immobilizing on Poly (Gly)/CPE through silica sol–gel (SiSG) entrapment. CV measurements were employed in order to understand the feasibility of poly (Gly) as an electron carrier between the immobilized peroxidase and the surface of CPE. By using differential pulse voltammetry (DPV) the calibration curves of DA was obtained in the range of 15–865 μM. The limit of detection (LOD) and limit of quantification (LOQ) of DA was found to be 6 × 10−7 M and 2 × 10−6 M respectively. The apparent Michaelis–Menten constant (Kmapp) was found to be 0.5 mM and illustrated that the good biological activity of the fixed enzyme. Electrochemical impedance spectroscopy (EIS) results confirmed the rapid electron transfer and also the immobilization of enzyme on the electrode surface. The biosensor showed high sensitivity, selectivity and reproducibility. This method has been used to determine DA in the presence of various interferences and in clinical preparations.

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Keywords
Carbon paste electrode; Horseradish peroxidase; Glycine; Multiwalled carbon nanotubes; Silica sol–gel; Dopamine
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A novel horseradish peroxidase biosensor towards the detection of dopamine: A voltammetric study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 57, 10 April 2014, Pages 8–15
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us