fulltext.study @t Gmail

Application of lipase immobilized on the biocompatible ternary blend polymer matrix for synthesis of citronellyl acetate in non-aqueous media: Kinetic modelling study

Paper ID Volume ID Publish Year Pages File Format Full-Text
17057 42637 2014 10 PDF Available
Title
Application of lipase immobilized on the biocompatible ternary blend polymer matrix for synthesis of citronellyl acetate in non-aqueous media: Kinetic modelling study
Abstract

•Compatible ternary blended matrix was made and applied for lipase immobilization.•Biocatalyst was studied for kinetics of the citronellyl acetate synthesis in detail.•Immobilized biocatalyst offered fivefold catalytic activity than aggregated lipase.•Reaction and kinetic studies showed that lipase followed ternary complex mechanism.•Protocol is recyclable and applied for greener synthesis of various flavour esters.

This work reports the use of new support for immobilization of lipase Burkholderia cepacia (BCL) matrix made up of polylactic acid (PLA), chitosan (CH), and polyvinyl alcohol (PVA). Initially lipase from various microbial sources and immobilization support composition was screened to obtain a robust biocatalyst. Among various biocatalysts preparation, the PLA:PVA:CH:BCL (1:6:1:2) was worked as a robust biocatalyst for the citronellyl acetate synthesis. Various reaction parameters were studied in detail to obtain the suitable reaction conditions for model citronellyl acetate synthesis reaction. Various kinetic parameters such as rmax, Ki(citronellol), Km(citronellol), Km(vinyl acetate) were determined using non-linear regression analysis for the ternary complex as well as bi–bi ping-pong mechanism. The experimental results and kinetic study showed that citronellyl acetate synthesis catalyzed by immobilized lipase BCL followed the ternary complex mechanism with inhibition by alcohol (citronellol). The energy of activation for citronellyl acetate synthesis was found to be lower for immobilized lipase (8.9 kcal/mol) than aggregated lipase (14.8 kcal/mol) enzyme. The developed biocatalyst showed four to fivefold higher catalytic activity and excellent recyclability (up to six cycles) than the aggregated lipase.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Burkholderia cepacia lipase immobilization; Compatible ternary blend; Polymer matrix; Citronellyl acetate; Ternary complex mechanism
First Page Preview
Application of lipase immobilized on the biocompatible ternary blend polymer matrix for synthesis of citronellyl acetate in non-aqueous media: Kinetic modelling study
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 57, 10 April 2014, Pages 16–25
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us