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Characterization of a heat-active archaeal β-glucosidase from a hydrothermal spring metagenome

Paper ID Volume ID Publish Year Pages File Format Full-Text
17061 42637 2014 7 PDF Available
Title
Characterization of a heat-active archaeal β-glucosidase from a hydrothermal spring metagenome
Abstract

•A thermostable archaeal β-glucosidase was recovered from a hot spring metagenome.•The enzyme showed optimal activity at 90 °C with 40% activity at 105 °C.•Pressure increased the stability of the highly active Bgl1 up to 7-fold at 90 °C.•The presence of aluminum chloride enhanced the catalytic performance.

Thermostable enzymes are required for application in a wide range of harsh industrial processes. High stability and activity at elevated temperatures, as well as high tolerances toward various reagents and solvents, are needed. In this work, a glycoside hydrolase family 1 β-glucosidase (Bgl1) of archaeal origin was isolated from a hydrothermal spring metagenome. The enzyme showed a broad substrate spectrum with activity toward cellobiose, cellotriose and lactose. Compared to most enzymes, extremely high specific activity with 3195 U/mg was observed at 90 °C and pH 6.5. Bgl1 was completely stable at pH 4.5–9.5 for 48 h at 4 °C. More than 40% of activity was measured at 105 °C. A thermal activation was observed at 90 °C after 30 min. Enzyme stability was enhanced (5- and 7-fold) after applying pressure of 100 and 200 bar at 90 °C for 2 h, respectively. The affinity of the β-glucosidase to its substrate was significantly increased in the presence of AlCl3. The Ki value for glucose was 150 mM. These distinctive characteristics distinguish Bgl1 from other enzymes described so far and make this enzyme suitable for application in numerous processes that run at high temperatures.

Keywords
Metagenomic library; Pressure; Thermostability; Aluminum chloride; Hyperthermophile
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Characterization of a heat-active archaeal β-glucosidase from a hydrothermal spring metagenome
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 57, 10 April 2014, Pages 48–54
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us