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Versatile peroxidase as a valuable tool for generating new biomolecules by homogeneous and heterogeneous cross-linking

Paper ID Volume ID Publish Year Pages File Format Full-Text
17087 42640 2013 9 PDF Available
Title
Versatile peroxidase as a valuable tool for generating new biomolecules by homogeneous and heterogeneous cross-linking
Abstract

•VP was checked for first time as biocatalyst in polymerization reactions.•The enzyme was efficient cross-linking molecules of different sizes and origins.•The polymerized substrates have relevance in different biotechnological processes.•VP was very stable in organic co-solvents as ethanol.•Novel molecules were obtained after heterogeneous cross-linking.

The modification and generation of new biomolecules intended to give higher molecular-mass species for biotechnological purposes, can be achieved by enzymatic cross-linking. The versatile peroxidase (VP) from Pleurotus eryngii is a high redox-potential enzyme with oxidative activity on a wide variety of substrates. In this study, VP was successfully used to catalyze the polymerization of low molecular mass compounds, such as lignans and peptides, as well as larger macromolecules, such as protein and complex polysaccharides. Different analytical, spectroscopic, and rheological techniques were used to determine structural changes and/or variations of the physicochemical properties of the reaction products. The lignans secoisolariciresinol and hydroxymatairesinol were condensed by VP forming up to 8 unit polymers in the presence of organic co-solvents and Mn2+. Moreover, 11 unit of the peptides YIGSR and VYV were homogeneously cross-linked. The heterogeneous cross-linking of one unit of the peptide YIGSR and several lignan units was also achieved. VP could also induce gelation of feruloylated arabinoxylan and the polymerization of β-casein. These results demonstrate the efficacy of VP to catalyze homo- and hetero-condensation reactions, and reveal its potential exploitation for polymerizing different types of compounds.

Keywords
Enzymatic polymerization; Organic co-solvent; Lignan; Peptide; β-Casein; Feruloylated arabinoxylan
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Versatile peroxidase as a valuable tool for generating new biomolecules by homogeneous and heterogeneous cross-linking
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 52, Issues 6–7, 10 May 2013, Pages 303–311
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us