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Highly efficient enzymatic preparation of c-di-AMP using the diadenylate cyclase DisA from Bacillus thuringiensis

Paper ID Volume ID Publish Year Pages File Format Full-Text
17089 42640 2013 6 PDF Available
Title
Highly efficient enzymatic preparation of c-di-AMP using the diadenylate cyclase DisA from Bacillus thuringiensis
Abstract

•DisA from Bacillus thuringiensis (btDisA) exerts high activity to synthesize c-di-AMP from ATP.•The whole process only incorporates enzymatic reaction, HPLC separation, and lyophilization.•HPLC separation can ensure the purity of c-di-AMP.•More than 100 mg of high pure c-di-AMP was obtained using 2 μM btDisA in a 50 mL reaction system.•The method is convenient to be scaled up and c-di-AMP has promising application prospects.

Cyclic 3′,5′-diadenosine monophosphate (c-di-AMP) is a newly recognized bacterial nucleotide second messenger molecule. In addition, it has been shown to be a potential vaccine adjuvant. Although multiple methods are available for c-di-AMP synthesis, the yields are low and the purification procedures are laborious. Here, we report an enzymatic method for more efficient and economical c-di-AMP synthesis using a diadenylate cyclase DisA from Bacillus thuringiensis BMB 171 (btDisA). After overexpression and purification of btDisA, the enzyme-catalyzed reaction conditions were further investigated. Under the optimum conditions, in which 100 mM CHES (pH 9.5) containing 2 μM btDisA, 10 mM ATP, and 10 mM MgCl2 was incubated at 50 °C for 4 h, a high conversion rate of c-di-AMP was obtained. Coupling this process with HPLC purification and lyophilization yielded 100 mg of highly pure c-di-AMP that was harvested in white powder form from a 50 mL enzyme-catalyzed reaction system. The protocol is not only directly applicable for preparing abundant amounts of c-di-AMP for extensive biochemical and immunological use, but can also be scaled up to meet the requirements for medical applications.

Keywords
C-di-AMP; Diadenylate cyclase; DisA; Enzymatic preparation; Bacillus thuringiensis
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Highly efficient enzymatic preparation of c-di-AMP using the diadenylate cyclase DisA from Bacillus thuringiensis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 52, Issues 6–7, 10 May 2013, Pages 319–324
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us