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Immobilization of glucose oxidase on chitosan-based porous composite membranes and their potential use in biosensors

Paper ID Volume ID Publish Year Pages File Format Full-Text
17098 42640 2013 7 PDF Available
Title
Immobilization of glucose oxidase on chitosan-based porous composite membranes and their potential use in biosensors
Abstract

The glucose oxidase (GOx) enzyme was immobilized on chitosan-based porous composite membranes using a covalent bond between GOx and the chitosan membrane. The chitosan-based porous membranes were prepared by the combination of the evaporation- and non-solvent-induced phase separation methods. To increase the membrane conductivity, carbon nanotubes (CNTs) were added to the chitosan solution. The resulting membranes were characterized in terms of water permeability, surface morphology and surface chemistry. Enzyme immobilization was performed on the chitosan membranes with and without activation using glutaraldehyde (GA). Three different configurations of working electrodes were evaluated to investigate the potential use of the modified membranes in biosensors. The results show that enzyme immobilization capacity was greater for membranes that had been activated than for membranes that had not been activated. In addition, activation increased the stability of the enzyme immobilization. The immobilization of GOx on chitosan-based membranes was influenced by both pH and the concentration of the enzyme solution. The presence of CNTs significantly increased the electrical conductivity of the chitosan membranes. The evaluation of three different configurations of working electrodes suggested that the third configuration, which was composed of an electrode-mediator-(chitosan and carbon nanotube) structure and enzyme, is the best candidate for biosensor applications.

► We prepared chitosan-based composite membrane via phase separation method. ► We immobilized GOx enzyme using chitosan-based composite membranes. ► We studied the effect of process parameter on binding capacity. ► We incorporated carbon nanotubes to increase the membrane conductivity and studied their effect on enzyme immobilization. ► We evaluated three different configurations of working electrodes for potential use in biosensors.

Keywords
Enzyme immobilization; Glucose oxidase; Glucose biosensor; Chitosan membrane
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Immobilization of glucose oxidase on chitosan-based porous composite membranes and their potential use in biosensors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 52, Issues 6–7, 10 May 2013, Pages 386–392
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us