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Characterization of Xyn30A and Axh43A of Bacillus licheniformis SVD1 identified by its genomic analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
17103 42641 2012 7 PDF Available
Title
Characterization of Xyn30A and Axh43A of Bacillus licheniformis SVD1 identified by its genomic analysis
Abstract

The genome sequence of Bacillus licheniformis SVD1, that produces a cellulolytic and hemi-cellulolytic multienzyme complex, was partially determined, indicating that the glycoside hydrolase system of this strain is highly similar to that of B. licheniformis ATCC14580. All of the fifty-six genes encoding glycoside hydrolases identified in B. licheniformis ATCC14580 were conserved in strain SVD1. In addition, two new genes, xyn30A and axh43A, were identified in the B. licheniformis SVD1 genome. The xyn30A gene was highly similar to Bacillus subtilis subsp. subtilis 168 xynC encoding for a glucuronoarabinoxylan endo-1,4-β-xylanase. Xyn30A, produced by a recombinant Escherichia coli, had high activity toward 4-O-methyl-d-glucurono-d-xylan but showed definite activity toward oat-spelt xylan and unsubstituted xylooligosaccharides. Recombinant Axh43A, consisting of a family-43 catalytic module of the glycoside hydrolases and a family-6 carbohydrate-binding module (CBM), was an arabinoxylan arabinofuranohydrolase (α-l-arabinofuranosidase) classified as AXH-m23 and capable of releasing arabinosyl residues, which are linked to the C-2 or C-3 position of singly substituted xylose residues in arabinoxylan or arabinoxylan oligomers. The isolated CBM polypeptide had an affinity for soluble and insoluble xylans and removal of the CBM from Axh43A abolished the catalytic activity of the enzyme, indicating that the CBM plays an essential role in hydrolysis of arabinoxylan.

► Genomic analysis of Bacillus licheniformis identified novel genes, xyn30A and axh43A. ► Xyn30A is a glucuronoarabinoxylan endo-1,4-β-xylanase. ► Axh43A is an arabinoxylan arabinofuranohydrolase (AXH-m) highly active toward arabinoxylan polymer and oligosaccharides. ► Axh43A comprises a family-43 catalytic module of the glycoside hydrolases and a CBM6. ► CBM6 having an affinity for arabinoxylan plays an essential role in its hydrolysis.

Keywords
Arabinofuranosidase; Bacillus licheniformis; Genome; Multienzyme complex; Xylanase
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Characterization of Xyn30A and Axh43A of Bacillus licheniformis SVD1 identified by its genomic analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issue 4, 10 September 2012, Pages 193–199
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us