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Production of resveratrol from tyrosine in metabolically engineered Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
17105 42641 2012 6 PDF Available
Title
Production of resveratrol from tyrosine in metabolically engineered Saccharomyces cerevisiae
Abstract

Resveratrol, a polyphenol compound found in grape skins, has been proposed to account for the beneficial effects of red wine against heart disease. To produce resveratrol in Saccharomyces cerevisiae, four heterologous genes were introduced: the phenylalanine ammonia lyase gene from Rhodosporidium toruloides, the cinnamic acid 4-hydroxylase and 4-coumarate:coenzyme A ligase genes both from Arabidopsis thaliana, and the stilbene synthase gene from Arachis hypogaea. When this recombinant yeast was cultivated by batch fermentation in YP medium containing 2% galactose, it produced 2.6 mg/L p-coumaric acid and 3.3 mg/L resveratrol. In order to increase the pool of malonyl-CoA, a key precursor in resveratrol biosynthesis, the acetyl-CoA carboxylase (ACC1) gene was additionally overexpressed in the yeast by replacing the native promoter of the ACC1 gene with the stronger GAL1 promoter and this resulted in enhanced production of resveratrol (4.3 mg/L). Furthermore, when tyrosine was supplemented in the medium, the concentration of resveratrol increased up to 5.8 mg/L. This result illustrates a possible strategy for developing metabolically engineered yeast strain for the economical production of resveratrol from cheap amino acids.

► p-Coumaric acid of 10.5 mg/L produced in recombinant yeast expressing PAL and C4H. ► Resveratrol of 3.4 mg/L produced from tyrosine in yeast introducing four genes. ► Production of resveratrol improved to 4.3 mg/L by increasing pool of malonyl-CoA. ► Addition of tyrosine led to increase in resveratrol produced up to 5.8 mg/L.

Keywords
Resveratrol; Saccharomyces cerevisiae; Phenylalanine ammonia lyase (PAL); Cinnamic acid 4-hydroxylase (C4H); 4-Coumarate:coenzyme A ligase (4CL1); Stilbene synthase (STS)
First Page Preview
Production of resveratrol from tyrosine in metabolically engineered Saccharomyces cerevisiae
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issue 4, 10 September 2012, Pages 211–216
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering