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Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis

Paper ID Volume ID Publish Year Pages File Format Full-Text
17107 42641 2012 6 PDF Available
Title
Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis
Abstract

The green alga Haematococcus pluvialis can produce large amounts of pink carotenoid astaxanthin which is a high value ketocarotenoid. In our study, transcriptional expression patterns of eight carotenoid genes in H. pluvialis in response to SA were measured using qRT-PCR. Results indicated that both 25 and 50 mg/L salicylic acid (SA) could increase astaxanthin productivity and enhance transcriptional expression of eight carotenoid genes in H. pluvialis. But these genes exhibited different expression profiles. Moreover, SA25 (25 mg/L SA) induction had a greater effect on the transcriptional expression of ipi-1, psy, pds, crtR-B and lyc (more than 6-fold up-regulation) than on ipi-2, bkt and crtO, but SA50 (50 mg/L SA) treatment had a greater impact on the transcriptional expression of ipi-1, ipi-2, pds, crtR-B and lyc than on psy, bkt and crtO. Furthermore, astaxanthin biosynthesis under SA was up-regulated mainly by ipi-1, ipi-2, psy, crtR-B, bkt and crtO at transcriptional level, lyc at post-transcriptional level and pds at both levels. Summarily, these results suggest that SA constitute molecular signals in the network of astaxanthin biosynthesis. Induction of astaxanthin accumulation by SA without any other stimuli presents an attractive application potential in astaxanthin production with H. pluvialis.

► Astaxanthin accumulation by H. pluvialis was induced effectively by SA. ► All eight carotenogenic genes were up-regulated by SA treatments. ► Carotenogenic genes exhibited different mRNA expression profiles when exposed to SA. ► Surface of alga cells changed drastically along with astaxanthin accumulation.

Keywords
ABA, abscisic acid; bkt, carotenoid ketolase gene; crtO, carotenoid oxygenase gene; crtR-B, carotenoid hydroxylase gene; ipi, isopentenyl diphosphate isomerase gene; JA, jasmonic acid; lyc, lycopene β-cyclase gene; MJ, methyl jasmonate; pds, phytoene desa
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Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issue 4, 10 September 2012, Pages 225–230
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us