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Activation of immobilized enzymes by acoustic wave resonance oscillation

Paper ID Volume ID Publish Year Pages File Format Full-Text
17114 42643 2014 5 PDF Available
Title
Activation of immobilized enzymes by acoustic wave resonance oscillation
Abstract

•Oxidase enzymes were immobilized on a ferroelectric Pb(Zr, Ti)O3 crystal.•The crystal generated acoustic wave resonance oscillation by rf electric power.•The oscillation remarkably increased the enzyme activity for substrate oxidation.•The enhanced activity was nearly preserved even after oscillation-off.•The oscillation enhanced the interactions between the enzyme and substrates.

Acoustic wave resonance oscillation has been used successfully in the development of methods to activate immobilized enzyme catalysts. In this study, resonance oscillation effects were demonstrated for enzyme reactions on galactose oxidase (GAD), D-amino acid oxidase (DAAO), and L-amino acid oxidase (LAAO), all of which were immobilized covalently on a ferroelectric lead zirconate titanate (PZT) device that could generate thickness-extensional resonance oscillations (TERO) of acoustic waves. For galactose oxidation on immobilized GAD in a microreactor, TERO generation immediately increased enzyme activity 2- to 3-fold. Eliminating TERO caused a slight decrease in the activity, with ∼90% of the enhanced activity retained while the reaction proceeded. Contact of the enhanced enzyme with a galactose-free solution caused almost complete reversion of the activity to the original low level before TERO generation, indicating that, not only TERO-induced GAD activation, but also preservation of the increased activity, required a galactose substrate. Similar activity changes with TERO were observed for enzyme reactions on DAAO and LAAO. Kinetic analysis demonstrated that TERO helped strengthen the interactions of the immobilized enzyme with the reactant substrate and promoted formation of an activation complex.

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Keywords
Immobilized enzymes; Resonance oscillation; Acoustic waves; Galactose oxidase; Amino acid oxidases; Enzyme activation
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Activation of immobilized enzymes by acoustic wave resonance oscillation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 67, December 2014, Pages 27–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us