fulltext.study @t Gmail

Design of a novel chimeric tissue plasminogen activator with favorable Vampire bat plasminogen activator properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
17122 42643 2014 5 PDF Available
Title
Design of a novel chimeric tissue plasminogen activator with favorable Vampire bat plasminogen activator properties
Abstract

•We designed a chimeric t-PA according to b-PA properties and then expressing in Pichia pastoris.•The fibrin binding of new protein was 1.2 fold more than human full-length t-PA.•The new model showed 1560 folds higher catalytic activity in the presence of fibrin compared as fibrin was absent.•The new construct was 44% less inhibited by plasminogen activator inhibitor than human full-length t-PA.•This new chimeric t-PA possesses b-PA properties resulting in reduced side effects and prolonged half-life.

Fibrinolytic agents are widely used in treatment of the thromboembolic disorders. The new generations like recombinant tissue plasminogen activator (t-PA, alteplase) are not showing promising results in clinical practice in spite of displaying specific binding to fibrin in vitro. Vampire bat plasminogen activator (b-PA) is a plasminogen activator with higher fibrin affinity and specificity in comparison to t-PA resulting in reduced probability of hemorrhage. b-PA is also resistant to plasminogen activator inhibitor-1 (PAI-1) showing higher half-life compared to other variants of t-PA. However, its non-human origin was a driving force to design a human t-PA with favorable properties of b-PA. In the present study, we designed a chimeric t-PA with desirable b-PA properties and this new molecule was called as CT-b. The construct was prepared through kringle 2 domain removal and replacement of t-PA finger domain with b-PA one. In addition, the KHRR sequence at the initial part of protease domain was replaced by four alanine residues. The novel construct was integrated in Pichia pastoris genome by electroporation. Catalytic activity was investigated in the presence and absence of fibrin. The purified protein was analyzed by western blot. Fibrin binding and PAI resistance assays were also conducted. The activity of the recombinant protein in the presence of fibrin was 1560 times more than its activity in the absence of fibrin, showing its higher specificity to fibrin. The fibrin binding of CT-b was 1.2 fold more than t-PA. In addition, it was inhibited by PAI enzyme 44% less than t-PA. Although the presented data demonstrate a promising in vitro activity, more in vivo studies are needed to confirm the therapeutic advantage of this novel plasminogen activator.

Keywords
b-PA; Fibrin binding; Pichia pastoris; Catalytic activity; Plasminogen activator inhibitor-1 (PAI-1); Tissue plasminogen activator (t-PA)
First Page Preview
Design of a novel chimeric tissue plasminogen activator with favorable Vampire bat plasminogen activator properties
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 67, December 2014, Pages 82–86
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us