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Detoxification of sulfur mustard by enzyme-catalyzed oxidation using chloroperoxidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17125 42644 2013 7 PDF Available
Title
Detoxification of sulfur mustard by enzyme-catalyzed oxidation using chloroperoxidase
Abstract

•Chloroperoxidase (CPO) accelerates disposal of sulfur mustard from water solutions.•We optimized the conditions of enzymatic oxidation of sulfur mustard (SM).•We found that the enzymatic oxidation of SM is of a pseudo-first order.•Determined Michaelis constant for the system: CPO–SM–H2O2 is 1.87 × 10−3 mol dm−3.

One of the most interesting methods for the detoxification of sulfur mustard is enzyme-catalyzed oxidation. This study examined the oxidative destruction of a sulfur mustard by the enzyme chloroperoxidase (EC 1.11.1.10). Chloroperoxidase (CPO) belongs to a group of enzymes that catalyze the oxidation of various organic compounds by peroxide in the presence of a halide ion. The enzymatic oxidation reaction is affected by several factors: pH, presence or absence of chloride ion, temperature, the concentrations of hydrogen peroxide and enzyme and aqueous solubility of the substrate. The optimum reaction conditions were determined by analyzing the effects of all factors, and the following conditions were selected: solvent, Britton–Robinson buffer (pH = 3) with tert-butanol (70:30 v/v); CPO concentration, 16 U/mL; hydrogen peroxide concentration, 40 mmol/L; sodium chloride concentration, 20 mmol/L. Under these reaction conditions, the rate constant for the reaction is 0.006 s−1. The Michaelis constant, a measure of the affinity of an enzyme for a particular substrate, is 1.87 × 10−3 M for this system. The Michaelis constant for enzymes with a high affinity for their substrate is in the range of 10−5 to 10−4 M, so this value indicates that CPO does not have a very high affinity for sulfur mustard.

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Keywords
Chemical warfare agents; Decontamination of sulfur mustard; Catalysis; Chloroperoxidase; Enzymatic oxidation; Michaelis–Menten equation; Michaelis constant
First Page Preview
Detoxification of sulfur mustard by enzyme-catalyzed oxidation using chloroperoxidase
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 5, 10 October 2013, Pages 295–301
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering