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Application of anoxia with glucose addition for the enhanced production of hCTLA4Ig in transgenic rice suspension cell cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
17140 42646 2012 6 PDF Available
Title
Application of anoxia with glucose addition for the enhanced production of hCTLA4Ig in transgenic rice suspension cell cultures
Abstract

To enhance the production of hCTLA4Ig in transgenic rice suspension cell cultures, anoxic conditions were applied during the production phase. Under the anoxic conditions in sugar-depleted media, cell viability was reduced rapidly and protease activity increased compared to aerobic conditions. However, the maximum production level of hCTLA4Ig with sugar-depleted anoxic conditions was the same as that in aerobic conditions. In addition, the production of hCTLA4Ig under anoxic conditions reached a peak 2 days earlier than that in aerobic conditions. Addition of 30 mM glucose at the production phase under anoxic conditions markedly improved cell viability. A viability level over 65% could be maintained for more than 30 days. Repression of the RAmy3D promoter by residual sugar in the production of hCTLA4Ig was not observed under anoxic conditions with 30 mM glucose. In addition, the production periods of hCTLA4Ig was extended up to 30 days and the maximum production level of hCTLA4Ig under anoxic conditions was 2.1-fold higher. Therefore, anoxic conditions could be used for the enhanced production of hCTLA4Ig in transgenic rice cell cultures.

► We investigated the enhanced production of hCTLA4Ig in transgenic rice cell cultures by applying anoxia. ► Under the anoxic conditions with small amounts of glucose, cell viability was improved for 30 days. ► Repression of the RAmy3D promoter was not observed and the production periods could be extended. ► Maximum hCTLA4Ig production under anoxic conditions was 2.1-fold higher. ► Anoxic conditions could be used for the enhanced production of hCTLA4Ig in transgenic rice cell cultures.

Keywords
Anoxia; Transgenic rice cell cultures; hCTLA4Ig; RAmy3D promoter
First Page Preview
Application of anoxia with glucose addition for the enhanced production of hCTLA4Ig in transgenic rice suspension cell cultures
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issues 6–7, 10 May 2012, Pages 298–303
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering