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Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and α-amylase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17147 42646 2012 5 PDF Available
Title
Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and α-amylase
Abstract

A diploid yeast strain displaying both α-amylase and glucoamylase was developed for repeated fermentation from raw starch. First, the construct of α-amylase was optimized for cell surface display, as there have been no reports of α-amylase-displaying yeast. The modified yeast displaying both glucoamylase and α-amylase produced 46.5 g/l of ethanol from 200 g/l of raw corn starch after 120 h of fermentation, and this was 1.5-fold higher when compared to native α-amylase-displaying yeast. Using the glucoamylase and modified α-amylase co-displaying diploid strain, we repeated fermentation from 100 g/l of raw starch for 23 cycles without the loss of α-amylase or glucoamylase activity. The average ethanol productivity and yield during repeated fermentation were 1.61 g/l/h and 76.6% of the theoretical yield, respectively. This novel yeast may be useful for reducing the cost of bio-ethanol production and may be suitable for industrial-scale bio-ethanol production.

► We developed optimized α-amylase for yeast cell surface display. ► We developed glucoamylase and optimized α-amylase displaying diploid yeast. ► Repeated fermentation from raw starch was carried out using the diploid yeast. ► The ethanol productivity and yield were 1.61 g/l/h and 76.6%. ► Amylase activities and ethanol productivity were maintained for 23 cycles.

Keywords
Ethanol; Yeast; Amylase; Starch; Repeated fermentation
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Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and α-amylase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issues 6–7, 10 May 2012, Pages 343–347
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us