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Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
17168 42648 2014 8 PDF Available
Title
Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles
Abstract

•Metal ions chelated magnetic nanoparticles were successfully synthesized.•β-Glucosidase was successfully immobilized on magnetic nanoparticles.•The immobilized β-glucosidase showed enhanced activity and good reusability.•The immobilized β-glucosidase was promising in application.

A novel magnetic Fe3O4 nanoparticles (MNPs) coupled with agarose (AMNPs) was synthesized using co-precipitation via alkaline condition and span-80 surfactants in organic solvent. Iminodiacetate was first attached to the MNPs through epichlorohydrin agent and then chelated with metal ions. The morphology and chemical properties of these prepared supports were characterized by scanning electron microscopy (SEM), X-ray power diffraction (XRD), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FT-IR). Among them, the Co2+-chelated AMNPs (AMNPs-ECH-IDA-Co2+) showed the second highest enzyme adsorption capacity of 1.81 mg/g particles, and achieved the largest activity recovery of 117% per protein gram in immobilization of β-glucosidase (BGL). The Michaelis constant (Km) and Vmax of the immobilized BGL were 0.904 mM and 0.057 μmol/min, respectively, and its activation energy was much lower than the free form. Moreover, the immobilized enzyme exhibited enhanced thermostability and operational stability. It still retained more than 90% of its initial activity after being operated for 15 successive batches. This study demonstrates that the immobilized β-glucosidase has a good prospect in industrial applications.

Keywords
β-Glucosidase; Immobilization; Biocatalysis; Magnetic particles; Kinetic parameter
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Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 63, September 2014, Pages 50–57
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us