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Laccases from Aureobasidium pullulans ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
17177 42649 2013 5 PDF Available
Title
Laccases from Aureobasidium pullulans ☆
Abstract

•Aureobasidium pullulans strains were surveyed for laccase production.•Laccase production was strongly associated with pigment production.•Laccases were more thermostable than those from lignin-degrading fungi.•These enzymes could be useful for industrial or bioremediation applications.

Laccases are polyphenol oxidases (EC 1.10.3.2) that have numerous industrial and bioremediation applications. Laccases are well known as lignin-degrading enzymes, but these enzymes can play numerous other roles in fungi. In this study, 41 strains of the fungus Aureobasidium pullulans were examined for laccase production. Enzymes from A. pullulans were distinct from those from lignin-degrading fungi and associated with pigment production. Laccases from strains in phylogenetic clade 5, which produced a dark vinaceous pigment, exhibited a temperature optimum of 50–60 °C and were stable for an hour at 50 °C, unlike enzymes from the lignin-degrading fungi Trametes versicolor and Pycnoporus cinnabarinus. Laccase purified from A. pullulans strain NRRL 50381, a representative of clade 5, was glycosylated but had a molecular weight of 60–70 kDa after Endo H treatment. Laccase purified from strain NRRL Y-2568, which produced a dark olivaceous pigment, was also glycosylated, but had a molecular weight of greater than 100 kDa after Endo H treatment.

Keywords
Aureobasidium pullulans; Glycosylation; Laccase; Thermostability; Trametes versicolor; Pycnoporus cinnabarinus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 1, 10 June 2013, Pages 33–37
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us