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Evaluation of structure and hydrolysis activity of Candida rugosa Lip7 in presence of sub-/super-critical CO2

Paper ID Volume ID Publish Year Pages File Format Full-Text
17189 42650 2012 5 PDF Available
Title
Evaluation of structure and hydrolysis activity of Candida rugosa Lip7 in presence of sub-/super-critical CO2
Abstract

This work aimed to assess the effect of sub-/super-critical CO2 on the structure and activity of Candida rugosa Lip7 (CRL7) in its solution form. The structure was examined by SDS-PAGE gel electrophoresis, circular dichroism (CD) and fluorescence spectra photometry. Results revealed that the primary structure remained intact after sub-/super-critical CO2 treatment, and the secondary structure altered at the pressure of 10 MPa and temperature 40 °C for 30 min incubation, but it was reflex to its native form with increasing incubation time up to 150 min under 10 MPa and 40 °C. Meanwhile, the tertiary structure via fluorescence spectra analysis showed that the intensity of the maximal emission wavelength at 338 nm decreased under the conditions of 10 MPa and 40 °C for 150 min. Furthermore, the residue hydrolysis activity and kinetics constants (Vmax and Km) of CRL7 treated with sub-/super-critical CO2 were also investigated. In cases of 6 MPa and 35 °C, or 10 MPa and 40 °C for 30 min, activity variance of CRL7 was maybe caused by its secondary structure alteration. But in case of 10 MPa and 40 °C for 150 min, the tertiary structure change was perhaps responsibility for CRL7 activity enhancement.

► We examine the effect of sub-/super-critical CO2 on CRL7 structure and activity. ► The CRL7 primary structure remained intact after sub-/super-critical CO2 treatment. ► The secondary and tertiary structure were affected depending on conditions. ► The residue activity and kinetics constants of treated CRL7 were also investigated.

Keywords
Candida rugosa Lip7 (CRL7); Supercritical carbon dioxide (SC-CO2); Structure; Hydrolysis activity; Kinetics constants
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Evaluation of structure and hydrolysis activity of Candida rugosa Lip7 in presence of sub-/super-critical CO2
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issues 6–7, 10 December 2012, Pages 354–358
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us