fulltext.study @t Gmail

Boronate affinity chromatography of cells and biomacromolecules using cryogel matrices

Paper ID Volume ID Publish Year Pages File Format Full-Text
17192 42650 2012 9 PDF Available
Title
Boronate affinity chromatography of cells and biomacromolecules using cryogel matrices
Abstract

Boronate affinity chromatography involves the interaction between cis-diol containing molecules and the hydroxyl group of boronate. Boronate affinity based cryogel chromatography matrices have been developed and the ligands were immobilized by two methods i.e., grafting of the boronate ligand on to the matrix and by copolymerization of monomer containing boronate with other co-monomer. The boronate grafted cryogel column was used to capture adherent and non-adherent cells and the captured cells were recovered at different fructose concentrations as an eluting agent, in chromatography mode. It was found that the adherent cells could be recovered at relatively higher fructose concentration (0.5 M) than non-adherent cells which could be recovered by using low fructose concentration (0.1 M). This might be due to the difference in the content of glycoprotein in adherent and non-adherent cells. In this way a new separation method can be devised for the fractionation of adherent and non-adherent cells. In another study, a copolymerized boronate cryogel column was developed for the separation of RNA from the bacterial crude extract without any pre-processing. RNA molecules were specifically retained in the cryogel column due to interaction between 2,3′ diol group of ribose sugar in RNA and the hydroxyl group of boronate. The DNA molecules were passed through the column uninteracted due to absence of 2′-hydroxyl group. Later, bound RNA molecules were recovered from the boronate affinity cryogel column.

► Synthesis of boronate affinity macroporous monolithic cryogel matrices. ► Specific binding of RNA on cryogel matrix. ► Specific separation of adherent and nonadherent cells after binding to the boronate affinity cryogel column. ► Purification of total RNA from bacterial crude extract.

Keywords
Boronate affinity chromatography; Cryogel; Cell separation; RNA isolation; Adherent cells; Non adherent cellsAAPBA, acrylamidoaminophenyl boronic acid; APBA, aminophenyl boronic acid; DMA, dimethyl acrylamide; DNA, deoxyribose nucleic acid; HEMA, hydroxye
First Page Preview
Boronate affinity chromatography of cells and biomacromolecules using cryogel matrices
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issues 6–7, 10 December 2012, Pages 373–381
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us