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A protein from Pleurotus eryngii var. tuoliensis C.J. Mou with strong removal activity against the natural steroid hormone, estriol: Purification, characterization, and identification as a laccase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17196 42650 2012 6 PDF Available
Title
A protein from Pleurotus eryngii var. tuoliensis C.J. Mou with strong removal activity against the natural steroid hormone, estriol: Purification, characterization, and identification as a laccase
Abstract

A protein with strong removal activity against the natural estrogen estriol was purified from a culture supernatant of Pleurotus eryngii var. tuoliensis C.J. Mou. The protein was characterized as a laccase and had a molecular mass of 60 kDa on SDS-PAGE. The enzyme was most active at pH 7.0 and 50 °C. The partial N-terminal amino acid sequence of the enzyme showed homology with laccases from mushrooms, such as Pleurotus ostreatus, Coriolus versicolor (current name: Trametes versicolor), Pycnoporus cinnabarinus, and P. eryngii. A recombinant yeast assay confirmed that laccase treatment was very efficient for removing the estrogenic activity of steroid estrogens. Our results suggest that the enzyme may be applicable as a potential factor for removing natural steroid hormones.

► It had strong removal activity against natural estrogen estriol in a culture supernatant of Pleurotus eryngii var. touliensis. ► The purified protein was identified as a laccase. ► It was confirmed that the laccase was very efficient in removing the estrogenic activity. ► The enzyme can be applicable as a potential removal factor against natural steroid hormones. ► To our knowledge, this is the first report about the removal of natural estrogen using laccase from Pleurotus sp.

Keywords
Estrogenic activity; Estriol; Laccase; Pleurotus eryngii var. tuoliensis C.J. Mou
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A protein from Pleurotus eryngii var. tuoliensis C.J. Mou with strong removal activity against the natural steroid hormone, estriol: Purification, characterization, and identification as a laccase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issues 6–7, 10 December 2012, Pages 402–407
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us