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Glycosylated yellow laccases of the basidiomycete Stropharia aeruginosa

Paper ID Volume ID Publish Year Pages File Format Full-Text
17198 42651 2014 7 PDF Available
Title
Glycosylated yellow laccases of the basidiomycete Stropharia aeruginosa
Abstract

•S. aeruginosa secretes glycosylated yellow laccases Yel1p and Yel3p.•Yel1p and Yel3p have been purified and characterised.•Low coverage, high throughput transcriptome sequencing identified the Yel1 gene.•Theories about the differences between yellow and blue laccases are discussed.

Here we describe the identification, purification and characterisation of glycosylated yellow laccase proteins from the basidiomycete fungus Stropharia aeruginosa. Biochemical characterisation of two yellow laccases, Yel1p and Yel3p, show that they are both secreted, monomeric, N-glycosylated proteins of molecular weight around 55 kDa with substrate specificities typical of laccases, but lacking the absorption band at 612 nm typical of the blue laccase proteins. Low coverage, high throughput 454 transcriptome sequencing in combination with inverse-PCR was used to identify cDNA sequences. One of the cDNA sequences has been assigned to the Yel1p protein on the basis of identity between the translated protein sequence and the peptide data from the purified protein, and the full length gene sequence has been obtained. Biochemical properties, substrate specificities and protein sequence data have been used to discuss the unusual spectroscopic properties of S. aeruginosa proteins in the context of recent theories about the differences between yellow and blue laccases.

Keywords
ABTS 2,2′, azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); l-DOPA, L-3,4-dihydroxyphenylalanine; ConA, Concanavalin A; CBS, Centraalbureau voorLaccase; Yellow laccase; 454 pyrosequencing; Gene isolation; Inverse PCR; Dye decolorisation
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Glycosylated yellow laccases of the basidiomycete Stropharia aeruginosa
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volumes 58–59, 10 May 2014, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us