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Power generation from cellulose using mixed and pure cultures of cellulose-degrading bacteria in a microbial fuel cell

Paper ID Volume ID Publish Year Pages File Format Full-Text
17216 42652 2012 5 PDF Available
Title
Power generation from cellulose using mixed and pure cultures of cellulose-degrading bacteria in a microbial fuel cell
Abstract

Microbial fuel cells (MFCs) have been used to generate electricity from various organic compounds such as acetate, glucose, and lactate. We demonstrate here that electricity can be produced in an MFC using cellulose as the electron donor source. Tests were conducted using two-chambered MFCs, the anode medium was inoculated with mixed or pure culture of cellulose-degrading bacteria Nocardiopsis sp. KNU (S strain) or Streptomyces enissocaesilis KNU (K strain), and the catholyte in the cathode compartment was 50 mM ferricyanide as catholyte. The power density for the mixed culture was 0.188 mW (188 mW/m2) at a current of 0.5 mA when 1 g/L cellulose was used. However, the power density decreased as the cellulose concentration in the anode compartment decreased. The columbic efficiencies (CEs) ranged from 41.5 to 33.4%, corresponding to an initial cellulose concentration of 0.1–1.0 g/L. For the pure culture, cellobioase enzyme was added to increase the conversion of cellulose to simple sugars, since electricity production is very low. The power densities for S and K strain pure cultures with cellobioase were 162 mW/m2 and 145 mW/m2, respectively. Cyclic voltammetry (CV) experiments showed the presence of peaks at 380, 500, and 720 mV vs. Ag/AgCl for the mixed bacterial culture, indicating its electrochemical activity without an external mediator. Furthermore, this MFC system employs a unique microbial ecology in which both the electron donor (cellulose) and the electron acceptor (carbon paper) are insoluble.

► Electricity can be produced from cellulose as a substrate. ► Mixed culture of cellulose degrading bacteria are more efficient than pure culture bacteria. ► Cellobioase enzyme increased the power density when pure culture bacteria used in anode chamber. ► The power density decreased by increasing initial cellulose concentration.

Keywords
Microbial fuel cells; Cellulose; Electricity generation; Mixed bacterial culture; Cellobioase
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Power generation from cellulose using mixed and pure cultures of cellulose-degrading bacteria in a microbial fuel cell
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issue 5, 10 October 2012, Pages 269–273
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us