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Immobilization of horseradish peroxidase in phospholipid-templated titania and its applications in phenolic compounds and dye removal

Paper ID Volume ID Publish Year Pages File Format Full-Text
17223 42653 2014 6 PDF Available
Title
Immobilization of horseradish peroxidase in phospholipid-templated titania and its applications in phenolic compounds and dye removal
Abstract

•HRP was encapsulated in titania particles through biomimetic titanification process.•The stabilities of HRP were obviously improved by encapsulation in titania.•The encapsulated HRP can be used in removal of dye and phenolic compounds.•The encapsulated HRP showed better removal efficiency than free HRP.•The encapsulated HRP showed a moderately good capability of reutilization.

In this study, horseradish peroxidase (HRP) was encapsulated in phospholipid-templated titania particles through the biomimetic titanification process and used for the treatment of wastewater polluted with phenolic compounds and dye. The encapsulated HRP exhibited improved thermal stability, a wide range of pH stability and high tolerance against inactivating agents. It was observed an increase in Km value for the encapsulated HRP (8.21 mM) when compared with its free counterpart. For practical applications in the removal of phenolic compounds and dye by the encapsulated HRP, the removal efficiency for phenol, 2-chlorophenol, Direct Black-38 were 92.99%, 87.97%, and 79.72%, respectively, in the first treatment cycle. Additionally, the encapsulated HRP showed better removal efficiency than free HRP and a moderately good capability of reutilization.

Keywords
HRP, horseradish peroxidaseHorseradish peroxidase; Phospholipid-templated titania; Enzyme encapsulation; Phenolic compound; Dye
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Immobilization of horseradish peroxidase in phospholipid-templated titania and its applications in phenolic compounds and dye removal
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 55, 5 February 2014, Pages 1–6
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us