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Modification of glucose oxidase for the development of biocatalytic solvent inks

Paper ID Volume ID Publish Year Pages File Format Full-Text
17226 42653 2014 5 PDF Available
Title
Modification of glucose oxidase for the development of biocatalytic solvent inks
Abstract

•Glucose oxidase (A. niger) was modified using hydrophobic ion pairing.•Modification induced solubility of the enzyme in toluene.•Single enzyme composites with a mean diameter of 12.5 nm were formed.•Modification resulted in no significant change in K′mK′m and a decrease in k′catk′cat.•Modification allowed for direct printing on PET using piezoelectric inkjet printing.

Inkjet printing of enzymes onto hydrophobic polymeric material offers the potential for economical rapid deposition and patterning of biocatalysts for biosensor, microarray, and intelligent packaging applications. Non-polar solvent based inks provide simple vehicles for direct printing on these materials; however, enzymes are not readily soluble in such inks. Glucose oxidase (Aspergillus niger) was made soluble in toluene by hydrophobic ion pairing with didodecyldimethylammonium bromide. Following modification, single enzyme composites with a mean diameter of 12.5 nm were formed. The enzymes showed no significant change in K′mK′m and a 46% decrease in k′catk′cat compared to the native enzyme. Modification allowed for direct printing and patterning on PET using piezoelectric inkjet printing. Specific activity of the modified enzyme was reduced from 889 × 103 μmol/min/g to 2 × 103 μmol/min/g after printing. These results suggest that direct inkjet printing of enzymes onto hydrophobic polymers may be accomplished using enzyme modification as a means to induce solubility in solvent inks.

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Keywords
Ion pairing; Glucose oxidase; Inkjet printing; Immobilization; Deposition; Nanocomposites
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Modification of glucose oxidase for the development of biocatalytic solvent inks
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 55, 5 February 2014, Pages 21–25
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us