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New food for an old mouth: New enzyme for an ancient archaea

Paper ID Volume ID Publish Year Pages File Format Full-Text
17231 42653 2014 7 PDF Available
Title
New food for an old mouth: New enzyme for an ancient archaea
Abstract

•Contrary to literature, the presence of GSH and GSTs were shown in archaea.•Amounts of GSH were measured in Haloarcula hispanica.•H. hispanica GSTs specific activities were determined as 19.68 nmol min−1 mg−1 protein.•Vmax and Km values for H. hispanica GST activity toward CDNB and GSH were calculated.•It is the first report on this area.

As a multifunctional group of enzymes, glutathione S-transferases (GSTs) are capable of inactivation, degradation or excretion of wide range of compounds catalytically or non-catalytically. However, to date, no study has been addresses the presence of GSTs in archaea based on their enzymatic functions. In this study, beside glutathione (GSH) amount measurement, the determination of GST activity in halophilic archaeon called Haloarcula hispanica ATCC 33960 were aimed. According to the results, specific activity was determined as 19.68 nmol min−1 mg−1 protein and GSH content were found to be as 194 μg g−1 Km and Vmax values for CDNB and GSH calculated from Lineweaver-Burk plot were 0.46 mM and 27.93 nmol min−1 mg−1, 0.13 mM and 22.03 nmol min−1 mg−1, respectively. Hanes-Woolf and Eadie-Hofstee plots for CDNB and GSH were also found to be in co-relation with the results obtained from Lineweaver-Burk plot. To the best of our knowledge, GST enzymes have not been identified in archaea yet, at least based on their catalytic activities. Therefore, it is the first report on this area.

Keywords
Archaea; Glutathione; Glutathione S-transferase; Haloarcula hispanica; Optimization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 55, 5 February 2014, Pages 58–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us