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Enzyme activity assay for horseradish peroxidase encapsulated in peptide nanotubes

Paper ID Volume ID Publish Year Pages File Format Full-Text
17249 42654 2012 5 PDF Available
Title
Enzyme activity assay for horseradish peroxidase encapsulated in peptide nanotubes
Abstract

Encapsulation of horseradish peroxidase (HRP) inside a peptide nanotube (PNT) was demonstrated and its activity was measured. Enzyme assay verified that 0.16 μg of the enzymes were encapsulated in 1 mg of PNTs. The encapsulation was also verified with TEM, UV–vis spectroscopy, and FTIR. The activity of the encapsulated HRP was examined for thermal stability, long-term storage stability, and resistance to a denaturant. They showed good storage stability, retaining its activity up to 90%, while the free HRP lost 50% of its activity over the course of 18 days. At 55 °C, the encapsulated HRP activity remained 20% higher than that of the free HRP. With the denaturant, guanidinium hydrochloride (GdmHCl), the encapsulated HRP activity was maintained around 10% higher than the free HRP. This result proves that the encapsulation of HRP inside the PNT may be an effective way to keep the enzyme activity stable in various environments.

► Peptide nanotube (PNT) was used for encapsulating horseradish peroxidase. ► Evidence of enzyme encapsulation was proved by ABTS enzyme assay and STEM. ► UV and FTIR spectroscopies indicated sustained enzyme conformation with the PNT. ► The encapsulated enzyme showed superior stabilities of enzyme activity. ► The PNT-encapsulated enzyme showed enhanced stability against a denaturant.

Keywords
Peptide nanotube; Encapsulation; Horseradish peroxidase; Enzyme stability
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Enzyme activity assay for horseradish peroxidase encapsulated in peptide nanotubes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issue 2, 15 July 2012, Pages 81–85
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us