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Magnetic isolation of particles suspended in synovial fluid for diagnostics of natural joint chondropathies

Paper ID Volume ID Publish Year Pages File Format Full-Text
1725 89 2010 9 PDF Available
Title
Magnetic isolation of particles suspended in synovial fluid for diagnostics of natural joint chondropathies
Abstract

Millions of people are stricken with the degenerative joint disease known as osteoarthritis. Osteoarthritis is associated with biochemical and mechanical processes, and is characterized by loss of articular cartilage and hypertrophy of bone. As cartilage and bone particles are released into the synovial fluid, a variety of biomarkers have been suggested for the analysis of this fluid. Here we have developed a method for isolating bone and cartilage wear particles suspended in the synovial fluid of the hip, knee and ankle joints of humans, based on specific magnetization of collagens I and II. Bio-ferrography is used to capture the particles on glass slides, allowing microscopic, chemical and statistical analyses. The relations between the level of the disease and the number, dimensions, shape and chemical composition of the particles were established. The method, which was found to be sensitive and reliable, can easily be extended to other applications, such as diagnosis of cancer and infectious diseases, determination of the efficacy of drugs or optimization of implants.

Keywords
Bio-ferrography; Osteoarthritis; Immunochemistry; Magnetic labeling; Wear debris
First Page Preview
Magnetic isolation of particles suspended in synovial fluid for diagnostics of natural joint chondropathies
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 11, November 2010, Pages 4430–4438
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering