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Kinetic mechanism of fuculose-1-phosphate aldolase from the hyperthermophilic Archaeon Methanococcus jannaschii

Paper ID Volume ID Publish Year Pages File Format Full-Text
17258 42655 2012 6 PDF Available
Title
Kinetic mechanism of fuculose-1-phosphate aldolase from the hyperthermophilic Archaeon Methanococcus jannaschii
Abstract

Fuculose-1-phosphate aldolase (FucA) is a useful biocatalyst with potential applications in chiral synthesis. In this study, the overall kinetic mechanism of FucA from the archaeon Methanococcus jannaschii was studied. The Km values of dihydroxyacetone phosphate (DHAP) and dl-glyceraldehyde were 0.09 and 0.74 mM, respectively. Dead-end inhibition by trimethyl phosphonoacetate and dl-threose were competitive and uncompetitive with respect to DHAP and dl-glyceraldehyde. Inhibition patterns obtained using reaction products were noncompetitive vs. DHAP and competitive vs. dl-glyceraldehyde. The equilibrium constant was 8.309 × 10−3 M as assessed by varying the [DHAP]/[product] ratio at a fixed dl-glyceraldehyde concentration and by measuring the change in DHAP concentration after equilibrium was reached. This constant is consistent with the Keq value obtained from 13C NMR (15.625 × 10−3 M). The resultant inhibition kinetics may suggest the insights of kinetic mechanism of the FucA catalyzed reaction.

► The overall kinetic mechanism of Methanococcus jannaschii FucA was studied. ► Dead-end inhibitions of FucA specific inhibitors were competitive to DHAP. ► In contrast, these inhibitors were uncompetitive to dl-glyceraldehyde. ► The assessed equilibrium kinetics was consistent with the 13C NMR data.

Keywords
Fuculose-1-phosphate aldolase; Methanococcus jannaschii; Product inhibition; Dead-end inhibition
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Kinetic mechanism of fuculose-1-phosphate aldolase from the hyperthermophilic Archaeon Methanococcus jannaschii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issues 4–5, 5 April 2012, Pages 209–214
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us