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Histology and research at the hard tissue–implant interface using Technovit 9100 New embedding technique

Paper ID Volume ID Publish Year Pages File Format Full-Text
1727 89 2010 9 PDF Available
Title
Histology and research at the hard tissue–implant interface using Technovit 9100 New embedding technique
Abstract

Calcified tissues, like bones and teeth, are among the most challenging tissues for histological research. However, especially with respect to dental or orthopaedic research, powerful histological techniques are necessary to study pathological conditions or traumatic injuries, and to investigate the molecular and cellular mechanisms of regeneration processes and functional recovery. The situation is even more complicated in orthopaedic research because here metallic implants or other devices made of various materials are often present, and the hard tissue–implant interface is of crucial interest in both biocompatibility and functional recovery research. After the cutting–grinding technique, embedding in technical resins is the most promising approach. Here we describe an optimized and standardized embedding and cutting technique using Technovit 9100 New. Using this technique, we are able to perform enzyme histochemistry, immunohistochemistry, a great variety of classical histological stains and even in situ hybridization.

Keywords
Histology; Tissue–implant interface; Magnesium; Biocompatibility; Biodegradable
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Histology and research at the hard tissue–implant interface using Technovit 9100 New embedding technique
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 11, November 2010, Pages 4447–4455
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us