fulltext.study @t Gmail

Characterization of potential elastase inhibitor-peptides regulated by a molecular switch for wound dressings applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
17271 42656 2012 8 PDF Available
Title
Characterization of potential elastase inhibitor-peptides regulated by a molecular switch for wound dressings applications
Abstract

Elastase plays an important role in wound healing process, degrading damaged tissue and allowing complete tissue recovery. The levels of human neutrophil elastase (HNE) are usually controlled by endogenous inhibitors. However, in the presence of high levels of elastase, like the ones present in chronic wounds, the inhibitors cannot overcome this overproduction and the enzyme starts to degrade the surrounding healthy tissue. In this work we report the development of a molecular switch to control the elastase activity in the exudate of non-healing chronic wounds. A peptide library was generated and screened in a microarray format for protein kinase-mediated phosphorylation. Two peptides were identified as casein kinase Iδ (CKI) substrates: KRCCPDTCGIKCL and its analogous peptide KRMMPDTMGIKML, with cysteine residues replaced by methionine residues. These peptides were studied in solution, both in the phosphorylated and non-phosphorylated forms as potential inhibitors for elastase. The obtained results show that the reversible process of phosphorylation/dephosphorylation results in differential inhibitory activity of the peptides. Thus the reversible process of phosphorylation/dephosphorylation can be used as a kind of molecular switch to control elastase activity. Degradation studies reveal that both the inhibitor-peptides and CKI are degraded by elastase. These results envisage the safe utilisation of these inhibitor-peptides together with CKI in the formulation of wound dressings.

► A peptide library was designed using peptide sequences derived from endogenous proteins. ► The screening of the library in a microarray format identified a robust hit, Pep4. ► Phosphorylation of Pep4 and Pep4M reduces their inhibitory activity towards elastase. ► Differential inhibition by phosphorylated/dephosphorylated peptides fine tune elastase inhibition process.

Keywords
SLPI, secretory leukocyte protease inhibitor; ECP, eosinophil cationic protein; SP-D, surfactant protein D; ESI, elastase specific inhibitor; HNE, human neutrophil elastase; PPE, porcine pancreatic elastase; MBP, myelin basic protein; CKIδ, casein kinase
First Page Preview
Characterization of potential elastase inhibitor-peptides regulated by a molecular switch for wound dressings applications
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issue 2, 10 February 2012, Pages 107–114
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us