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Photocrosslinked bovine serum albumin hydrogels with partial retention of esterase activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
17274 42656 2012 7 PDF Available
Title
Photocrosslinked bovine serum albumin hydrogels with partial retention of esterase activity
Abstract

Novel biohybrid hydrogels based on bovine serum albumin (BSA) were synthesized by one-pot photopolymerization of chemically modified protein in the presence of N,N′-methylenebisacrylamide (MBA) as cross-linking agent under mild conditions. Two batches of methacrylated albumins were prepared by treating the protein with different amounts of methacrylic anhydride (MAN) and the degree of substitution (DS) of primary amines was quantified via trinitrobenzesulfonic acid (TNBS) colorimetric assay. Hydrogels readily formed when a diluted buffered solution of the modified protein and MBA was exposed to LW-UV in the presence of 1-[4-(2-hydroxyethoxy)phenyl]-2-hydroxy-2-methyl-1-propan-1-one (Irgacure 2959) as the radical initiator. In contrast, no hydrogel was obtained in the absence of a polymerizable BSA, nor when the cross-linker, the radical initiator or UV light exposure was excluded from the reaction, suggesting the critical importance of the combined conditions for hydrogel formation. Hydrogels were characterized via scanning electron microscopy (SEM) and the swelling ratios were monitored at different pHs. The esterolytic activity of the novel biohybrid materials was quantitatively investigated via UV–vis spectroscopy by measuring the release of para-nitrophenol upon incubation with para-nitrophenyl acetate (p-NPA) substrate. The effect of the addition of acrylic acid co-monomer and of the monomer concentration in the catalytic activity and in the swelling behavior was also examined. Finally, the reusability of the materials following one round of catalysis was evaluated.

► BSA-derived hydrogels were prepared via photopolymerization of chemically modified protein. ► Hydrogel formation occurred via free-radical photoinitiated polymerization under mild conditions. ► Hydrogels partially retained esterase-like activity towards a model p-nitrophenyl ester substrate. ► Hydrogels were reusable following one round of catalysis.

Keywords
Biomimetic catalyst; BSA; Hydrogel; Photocrosslinked; Reusable catalyst
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Photocrosslinked bovine serum albumin hydrogels with partial retention of esterase activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issue 2, 10 February 2012, Pages 130–136
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us