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Production of α-ketoisocaproate via free-whole-cell biotransformation by Rhodococcus opacus DSM 43250 with l-leucine as the substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
17279 42657 2011 5 PDF Available
Title
Production of α-ketoisocaproate via free-whole-cell biotransformation by Rhodococcus opacus DSM 43250 with l-leucine as the substrate
Abstract

This work aims to produce α-ketoisocaproate (KIC) from l-leucine via the free-whole-cell biotransformation of Rhodococcus opacus DSM 43250. The effects of temperature, pH, substrate concentration, cell concentration, and rotating speed on KIC production were examined. Furthermore, the biotransformation conditions were optimized with response surface methodology (RSM). The optimal biotransformation conditions were as follows: temperature 43.7 °C, pH 8.4, l-leucine concentration 5.1 g/L, cell concentration 30.4 g/L, and rotating speed 170 rpm. The maximal KIC production predicted by RSM model reached 1275 mg/L at the optimal conditions, and in the validated experiments, the maximal KIC production reached 1264 mg/L, which was very close to the predicted KIC production. The current work provides an alternative for the production of KIC and the results obtained here are useful for the biotransformatic KIC production on a large scale.

Keywords
α-Ketoisocaproate; Rhodococcus opacus DSM 43250; Free-whole-cell; Biotransformation; Response surface methodology; Optimization
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Production of α-ketoisocaproate via free-whole-cell biotransformation by Rhodococcus opacus DSM 43250 with l-leucine as the substrate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issue 4, 10 September 2011, Pages 321–325
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us