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Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: A model system for cellulase–poly-phenol interactions

Paper ID Volume ID Publish Year Pages File Format Full-Text
17282 42657 2011 7 PDF Available
Title
Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: A model system for cellulase–poly-phenol interactions
Abstract

Addition of non-ionic surfactants (NIS) is known to accelerate enzymatic lignocellulose hydrolysis. The mechanism behind this accelerating effect is still not elucidated but has been hypothesized to originate from favorable NIS–lignin interactions which alleviate non-productive adsorption of cellulases to lignin. In the current work we address this hypothesis using tannic acid (TAN) as a general poly-phenolic model compound (for lignin and soluble phenolics) and measure the mutual interactions of cellulases (CBHI, CBHII, EGI, EGII and BG), TAN and NIS (Triton X-100) using isothermal titration calorimetry (ITC). The experimental results suggest rather strong enzyme-specific interactions with TAN in reasonable agreement with enzyme specific lignin inhibition found in the literature. Enzyme–TAN interactions were disrupted by the presence of NIS by a mechanism of strong TAN–NIS interaction. The presence of NIS also alleviated the inhibitory effect of TAN on cellulase activity. All together the current work provides strong indications that favorable NIS–poly-phenol interactions alleviate non-productive cellulase–poly-phenol interactions and hence may provide a mechanism for the accelerating effect of NIS on lignocellulose hydrolysis.

Keywords
NIS, non-ionic surfactants; TAN, tannic acid; PCS, pretreated corn stover; CBHI, cellobiohydrolase I; CBHII, cellobiohydrolase II; EGI, endoglycosidase I; EGII, endoglycosidase II; BG, beta-glycosidase; PEG, poly-ethyleneglycol; CMC, critical micelle conc
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Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: A model system for cellulase–poly-phenol interactions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issue 4, 10 September 2011, Pages 353–359
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us