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In situ extractive fermentation for the production of hexanoic acid from galactitol by Clostridium sp. BS-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
17297 42658 2013 9 PDF Available
Title
In situ extractive fermentation for the production of hexanoic acid from galactitol by Clostridium sp. BS-1
Abstract

Clostridium sp. BS-1 produces hexanoic acid as a metabolite using galactitol and enhanced hexanoic acid production was obtained by in situ extractive fermentation with Clostridium sp. BS-1 under an optimized medium composition. For medium optimization, five ingredients were selected as variables, and among them yeast extract, tryptone, and sodium butyrate were selected as significant variables according to a fractional factorial experimental design, a steepest ascent experimental design, and a Box–Behnken experimental design. The optimized medium had the following compositions in modified Clostridium acetobutyricum (mCAB) medium: 15.5 g L−1 of yeast extract, 10.13 g L−1 of tryptone, 0.04 g L−1 of FeSO4·7H2O, 0.85 g L−1 of sodium acetate, and 6.47 g L−1 of sodium butyrate. The predicted concentration of hexanoic acid with the optimized medium was 6.98 g L−1, and this was validated experimentally by producing 6.96 g L−1 of hexanoic acid with Clostridium sp. BS-1 under the optimized conditions. In situ extractive fermentation for hexanoic acid removal was then applied in a batch culture system with the optimized medium and 10% (v/v) alamine 336 in oleyl alcohol as an extractive solvent. The pH of the culture in the extractive fermentation was maintained at 5.4–5.6 by an acid balance between production and retrieval by extraction. During a 16 day culture, the hexanoic acid concentration in the solvent increased to 32 g L−1 while it was maintained in a range of 1–2 g L−1 in the medium. The maximum rate of hexanoic acid production was 0.34 g L−1 h−1 in in situ extractive fermentation.

► Hexanoic acid was produced by Clostridium sp. BS-1 from galactitol, converted seaweed carbohydrate. ► In situ extractive fermentation was applied for the production of hexanoic acid by Clostridium sp. BS-1. ► The maximum rate of hexanoic acid production by Clostridium sp. BS-1 was 6.68 g L−1 day−1 in in situ extractive fermentation.

Keywords
Hexanoic acid; Clostridium sp. BS-1; Fractional factorial experimental design; Box–Behnken experimental design; In situ extractive fermentation; Galactitol
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In situ extractive fermentation for the production of hexanoic acid from galactitol by Clostridium sp. BS-1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 3, 15 August 2013, Pages 143–151
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us