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Transposition of IS elements induced by electroporation of suicide plasmid in Acidithiobacillus caldus

Paper ID Volume ID Publish Year Pages File Format Full-Text
17301 42658 2013 5 PDF Available
Title
Transposition of IS elements induced by electroporation of suicide plasmid in Acidithiobacillus caldus
Abstract

•Gene inactivation by ISAtc2 transposition was firstly discovered in A. caldus.•The features of ISAtc2 were identified.•The transposition frequency of ISAtc2 was ranged from 4% to 7%.•No reverse mutation occurred in the mutants within 50 generations.•IS elements may regulate the gene expression and metabolic pathways.

Transposition insertional mutagenesis of the insertion sequences (IS elements) was discovered for the first time in Acidithiobacillus caldus (A. caldus), when A. caldus MTH-04 hsdM (type I restriction-modification system M-subunit) mutant was constructed by electroporation of a suicide plasmid. The IS element, specifically inserting into hsdM gene, was analyzed, identified, and named ISAtc2. The transposition frequency of ISAtc2 was ranged from 4% to 7%, and no reverse mutation occurred in the mutants after 50 generations of proliferation without selective pressure. These results revealed that transposition of IS elements on A. caldus chromosome could regulate the gene expression and metabolic pathways by gene inactivation, gene loss and gene acquisition. Therefore, the transposition of IS elements in A. caldus may be an important and unique regulation mechanism for adaptation to the living condition.

Keywords
Acidithiobacillus caldus; Electroporation; IS elements; Suicide plasmid; Transposition mutagenesis
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Transposition of IS elements induced by electroporation of suicide plasmid in Acidithiobacillus caldus
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 3, 15 August 2013, Pages 165–169
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us