fulltext.study @t Gmail

Investigation of protein expression profiles of erythritol-producing Candida magnoliae in response to glucose perturbation

Paper ID Volume ID Publish Year Pages File Format Full-Text
17303 42658 2013 7 PDF Available
Title
Investigation of protein expression profiles of erythritol-producing Candida magnoliae in response to glucose perturbation
Abstract

•Erythritol production by C. magnoliae is associated with osmotic stress.•We employed proteomic tools to identify differentially expressed proteins.•Five proteins had strong response to glucose perturbation during fermentation.•Expression patterns of identified proteins corresponded well with previous studies.

Protein expression patterns of an erythritol-producing yeast, Candida magnoliae, were analyzed to identify differentially expressed proteins in response to glucose perturbation. Specifically, wild type C. magnoliae was grown under high and low glucose conditions and the cells were harvested at both mid-exponential and erythritol production phases for proteomic studies. In order to analyze intracellular protein abundances from the harvested cells quantitatively, total intracellular proteins were extracted and applied to two-dimensional gel electrophoresis for separation and visualization of individual proteins. Among the proteins distributed in the range of pI 4–7 and molecular weight 29–97 kDa, five osmo-responsive proteins were drastically changed in response to glucose perturbation. Hsp60 (Heat-shock protein 60), transaldolase and NADH:quinone oxidoreductase were down-regulated under the high glucose condition and Bro1 (BCK1-like Resistance to Osmotic shock) and Eno1 (enolase1) were up-regulated. These proteins are directly or indirectly related with cellular stress response. Importantly, protein expression patterns of Hsp60, Bro1 and Eno1 were strongly correlated with previous studies identifying the proteins perturbed by osmotic stress for other organisms including Saccharomyces cerevisiae.

Keywords
Candida magnoliae; Erythritol; Osmo-responsive protein; Proteomics
First Page Preview
Investigation of protein expression profiles of erythritol-producing Candida magnoliae in response to glucose perturbation
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 3, 15 August 2013, Pages 174–180
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us