fulltext.study @t Gmail

Flow cytometric immunoassay using E. coli with autodisplayed Z-domains

Paper ID Volume ID Publish Year Pages File Format Full-Text
17304 42658 2013 8 PDF Available
Title
Flow cytometric immunoassay using E. coli with autodisplayed Z-domains
Abstract

•Flow cytometry was used for simultaneous detection of multiple analytes using E. coli cells.•E. coli cells with and without fluorescence in cytosol were used for multiple analyte detection.•E. coli cell with autodisplayed Z-domains was used for hyper sensitive immunoassay.•Flow cytometry based multiple detection was demonstrated with HBsAg and CRP as model analytes.

Recently, we reported a highly sensitive immunoassay using Escherichia coli cells with autodisplayed Z-domains. In this work, E. coli cells with autodisplayed Z-domains were applied to the flow-cytometry-based simultaneous detection of multiple analytes. The E. coli cells were doubly transfected to express a fluorescent protein (tdTomato) in the cytosol and the autodisplayed Z-domains on the outer membrane. By using E. coli cells with only the autodisplayed Z-domains, immunoassay of multiple analytes could be performed simultaneously on the same sample. Flow cytometry can be used to identify the immunoassay type by simultaneously detecting the fluorescence signal from the cytosol (tdTomato) and the fluorescence from the outer membrane, enabling the quantification of bound analytes after treatment with additional fluorescently labeled antibodies. To demonstrate the immunoassay of multiple analytes by using flow cytometry, human hepatitis B virus surface antigen (HBsAg) and C-reactive protein (CRP), a broad spectrum inflammation marker, were used as model analytes.

Keywords
Flow cytometry; Autodisplay; Z-domain; Immunoassay; Hepatitis B virus surface antigen; C-reactive protein
First Page Preview
Flow cytometric immunoassay using E. coli with autodisplayed Z-domains
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 3, 15 August 2013, Pages 181–188
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us