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Cadmium detection by a thermally responsive elastin copolymer with metal-binding functionality

Paper ID Volume ID Publish Year Pages File Format Full-Text
17305 42658 2013 5 PDF Available
Title
Cadmium detection by a thermally responsive elastin copolymer with metal-binding functionality
Abstract

•Elastin copolymer genes with metal-binding functionality were cloned and expressed.•Characteristics of the EC-ELPs were observed.•Cadmium binding affinity and thermal behavior of EC-ELPs were investigated.

Heavy metals are of great concern to environmental safety because of their adverse effects on the environment and human health, even at very low levels. In particular, cadmium and several cadmium-containing compounds are carcinogens and induce many types of cancer. Biological extracts of cadmium have been given greater attention recently because they are considered to be environmentally benign and economically acceptable. Among promising candidates, one emerging technology is the use of tunable, metal-binding biopolymers based on elastin-like polypeptides (ELPs). An ELP consists of the repeating pentapeptide of specific amino acids, Val-Pro-Gly-Xaa-Gly (where the “guest residue” Xaa is any amino except proline) that undergoes a reversible phase transition at a specific temperature (transition temperature, Tt). However, the ELP itself is relatively non-selective. A biopolymer with metal-binding domains that have stronger affinity, capacity, and selectivity would have distinct advantages. We investigated the use of a new generation of ELP biopolymers, EC18-ELP containing synthetic phytochelatin (EC), which is a metal-binding protein with a repetitive motif (Glu-Cys)nGly, as the metal-binding domain. In this study, an EC18-ELP fusion protein was expressed in Escherichia coli and the metal binding ability of EC to cadmium was examined quantitatively. In addition, transition temperature variation was analyzed when the fusion protein bound to cadmium.

Keywords
Synthetic phytochelatin (EC); Elastin-like polypeptide (ELP); Cadmium; Biosensor
First Page Preview
Cadmium detection by a thermally responsive elastin copolymer with metal-binding functionality
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 3, 15 August 2013, Pages 189–193
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering