fulltext.study @t Gmail

Assessment of long-term cryopreservation for production of hCTLA4Ig in transgenic rice cell suspension cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
17309 42658 2013 7 PDF Available
Title
Assessment of long-term cryopreservation for production of hCTLA4Ig in transgenic rice cell suspension cultures
Abstract

•We investigated the long-term cryopreservation for the production of hCTLA4Ig in transgenic rice cell cultures.•After cryopreservation for 5 years, cell growth and hCTLA4Ig production were similar to those of control cultures.•hCTLA4Ig productivity of cryopreserved cells for 5 years was 1.81-fold higher than that of the periodically subcultured cells.•An efficient and successful cell-banking system using long-term cryopreservation was successfully established in transgenic rice cell cultures.

For the commercialization of plant-made pharmaceuticals (PMPs) using transgenic plant cell cultures, the establishment of a cell-banking system has been known to be an essential process. Plant cells are traditionally maintained by repeated subcultures. However, this method has several problems including genetic instability of transformed cell lines, time- and cost-consuming. In this study, long-term cryopreserved rice suspension cells were firstly investigated for the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig). The cryopreserved cells for 5 years were regrowed to callus successfully and then suspended into the liquid medium. Consequently, the maximum cell mass and the hCTLA4Ig production were similar levels compared to those of the non-cryopreserved cells (control) even though hCTLA4Ig productivity was 1.7-fold higher than that of control. To further assess the level of improvements in hCTLA4Ig productivity in cryopreserved cells, hCTLA4Ig production profiles were statistically assessed between data of the cryopreserved cells for 5 years and annual data of non-cryopreserved cells maintained by subculture for 5 years. These results also indicate that hCTLA4Ig productivity in cryopreserved cells for 5 years was significantly increased (p-value: <0.001, 95% confidence interval) and it could be related to cell lysis resulting in release of hCTLA4Ig which was confirmed by the measurement of electrolyte leakage. In conclusion, we show that the long-term cryopreservation of transgenic rice cells was possible to support stable cell lines for the production of PMPs.

Keywords
Transgenic rice cell culture; RAmy3D promoter; hCTLA4Ig; Cell-banking system; Long-term cryopreservation
First Page Preview
Assessment of long-term cryopreservation for production of hCTLA4Ig in transgenic rice cell suspension cultures
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 53, Issue 3, 15 August 2013, Pages 216–222
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us