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A novel selectable marker based on Aspergillus niger arginase expression

Paper ID Volume ID Publish Year Pages File Format Full-Text
17318 42659 2012 6 PDF Available
Title
A novel selectable marker based on Aspergillus niger arginase expression
Abstract

Selectable markers are valuable tools in transforming asexual fungi like Aspergillus niger. An arginase (agaA) expression vector and a suitable arginase-disrupted host would define a novel nutritional marker/selection for transformation. The development of such a marker was successfully achieved in two steps. The single genomic copy of A. niger arginase gene was disrupted by homologous integration of the bar marker. The agaA disruptant was subsequently complemented by transforming it with agaA expression vectors. Both citA and trpC promoters were able to drive the expression of arginase cDNA. Such agaA+ transformants displayed arginase expression pattern distinct from that of the parent strain. The results are also consistent with a single catabolic route for arginine in this fungus. A simple yet novel arginine-based selection for filamentous fungal transformation is thus described.

► First report for targeted disruption of arginase (aga) gene in filamentous fungi. ► citA and trpC promoters employed for homologous expression of arginase in A. niger. ► Novel nutritional selection through arginase complementation is defined. ► The results conform to a single catabolic route for arginine in A. niger.

Keywords
Fungal transformation; Arginase (agaA) disruption; Nutritional markers; Aspergillus niger
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A novel selectable marker based on Aspergillus niger arginase expression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 51, Issue 1, 10 June 2012, Pages 53–58
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us