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Covalent immobilization of catalase onto spacer-arm attached modified florisil: Characterization and application to batch and plug-flow type reactor systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
17330 42660 2011 8 PDF Available
Title
Covalent immobilization of catalase onto spacer-arm attached modified florisil: Characterization and application to batch and plug-flow type reactor systems
Abstract

Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and glutaraldehyde + 6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations of catalase onto modified supports were optimized to improve the efficiency of the overall immobilization procedures. The Vmax values of catalase immobilized via glutaraldehyde (CIG) and catalase immobilized via glutaraldehyde + 6-amino hexanoic acid (CIG-6-AHA) were about 0.6 and 3.4% of free catalase, respectively. The usage of 6-AHA as a spacer arm caused about 40 folds increase in catalytic efficiency of CIG-6-AHA (8.3 × 105 M−1 s−1) as compared to that of CIG (2.1 × 104 M−1 s−1). CIG and CIG-6-AHA retained 67 and 35% of their initial activities at 5 °C and 71 and 18% of their initial activities, respectively at room temperature at the end of 6 days. Operational stabilities of CIG and CIG-6-AHA were investigated in batch and plug-flow type reactors. The highest total amount of decomposed hydrogen peroxide (TAD-H2O2) was determined as 219.5 μmol for CIG-6-AHA in plug-flow type reactor.

Keywords
Catalase; Covalent immobilization; Reactors; Spacer-arm
First Page Preview
Covalent immobilization of catalase onto spacer-arm attached modified florisil: Characterization and application to batch and plug-flow type reactor systems
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issues 6–7, 10 December 2011, Pages 547–554
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering