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Characterization and optimization of β-galactosidase immobilization process on a mixed-matrix membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
17335 42660 2011 9 PDF Available
Title
Characterization and optimization of β-galactosidase immobilization process on a mixed-matrix membrane
Abstract

β-Galactosidase is an important enzyme catalyzing not only the hydrolysis of lactose to the monosaccharides glucose and galactose but also the transgalactosylation reaction to produce galacto-oligosaccharides (GOS). In this study, β-galactosidase was immobilized by adsorption on a mixed-matrix membrane containing zirconium dioxide. The maximum β-galactosidase adsorbed on these membranes was 1.6 g/m2, however, maximal activity was achieved at an enzyme concentration of around 0.5 g/m2. The tests conducted to investigate the optimal immobilization parameters suggested that higher immobilization can be achieved under extreme parameters (pH and temperature) but the activity was not retained at such extreme operational parameters. The investigations on immobilized enzymes indicated that no real shift occurred in its optimal temperature after immobilization though the activity in case of immobilized enzyme was better retained at lower temperature (5 °C). A shift of 0.5 unit was observed in optimal pH after immobilization (pH 6.5 to 7). Perhaps the most striking results are the kinetic parameters of the immobilized enzyme; while the Michaelis constant (Km) value increased almost eight times compared to the free enzyme, the maximum enzyme velocity (Vmax) remained almost constant.

Keywords
β-Galactosidase; Mixed-matrix membrane; Enzyme; Immobilization; Specific activity; Biocatalytic membrane
First Page Preview
Characterization and optimization of β-galactosidase immobilization process on a mixed-matrix membrane
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 49, Issues 6–7, 10 December 2011, Pages 580–588
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering