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Engineered Escherichia coli capable of co-utilization of cellobiose and xylose

Paper ID Volume ID Publish Year Pages File Format Full-Text
17337 42661 2012 4 PDF Available
Title
Engineered Escherichia coli capable of co-utilization of cellobiose and xylose
Abstract

Natural ability to ferment the major sugars (glucose and xylose) of plant biomass is an advantageous feature of Escherichia coli in biofuel production. However, excess glucose completely inhibits xylose utilization in E. coli and decreases yield and productivity of fermentation due to sequential utilization of xylose after glucose. As an approach to overcome this drawback, E. coli MG1655 was engineered for simultaneous glucose (in the form of cellobiose) and xylose utilization by a combination of genetic and evolutionary engineering strategies. The recombinant E. coli was capable of utilizing approximately 6 g/L of cellobiose and 2 g/L of xylose in approximately 36 h, whereas wild-type E. coli was unable to utilize xylose completely in the presence of 6 g/L of glucose even after 75 hours. The engineered strain also co-utilized cellobiose with mannose or galactose; however, it was unable to metabolize cellobiose in the presence of arabinose and glucose. Successful cellobiose and xylose co-fermentation is a vital step for simultaneous saccharification and co-fermentation process and a promising step towards consolidated bioprocessing.

► This is the first strain of E. coli to efficiently co-utilize cellobiose and xylose for all ratios tested. ► This is a potentially important strain in simultaneous saccharification and co-fermentation process as it can utilize the major pentose and hexose derived from plant biomass simultaneously. ► This strain can be used for fermentation of both hardwood hydrolysate and softwood hydrolysate.

Keywords
Cellobiose metabolism; Xylose metabolism; Co-metabolism; Escherichia coli
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Engineered Escherichia coli capable of co-utilization of cellobiose and xylose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issue 1, 5 January 2012, Pages 1–4
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us