fulltext.study @t Gmail

Enhancing macrolide production in Streptomyces by coexpressing three heterologous genes

Paper ID Volume ID Publish Year Pages File Format Full-Text
17338 42661 2012 5 PDF Available
Title
Enhancing macrolide production in Streptomyces by coexpressing three heterologous genes
Abstract

Antibiotic production in Streptomyces can often be increased by introducing heterologous genes into strains that contain an antibiotic biosynthesis gene cluster. A number of genes are known to be useful for this purpose. We chose three such genes and cloned them singly or in combination under the control of the strong constitutive ermE* promoter into a ϕC31-derived integrating vector that can be transferred efficiently by conjugation from Escherichia coli to Streptomyces. The three genes are adpA, a global regulator from Streptomyces coelicolor, metK, encoding S-adenosylmethionine synthetase from S. coelicolor, and, VHbS, hemoglobin from Vitreoscilla. The substitutions with GC in VHbS was intended to convert codons from lower usage to higher, yet causing no change to the encoded amino acid. Plasmids containing either one of these genes or genes in various combinations were introduced into Streptomyces sp. FR-008, which produces the macrolide antibiotic FR-008-III (also known as candicidin D). The largest increase in FR-008-III production was achieved by the plasmid containing all three genes. This plasmid also increased avermectin production in Streptomyces avermitilis, and is likely to be generally useful for improving antibiotic production in Streptomyces.

► A plasmid for coexpressing three heterologous genes adpA, metK and VHbS was constructed. ► Optimization of the VHb codon usage increased FR-008-III production. ► Production of FR-008-III and avermectin was significantly improved by combination of three factors.

Keywords
Streptomyces sp. FR-008; Candicidin; Avermectin; S-adenosylmethionine synthetase; Vitreoscilla hemoglobin; Transcriptional activator AdpA
First Page Preview
Enhancing macrolide production in Streptomyces by coexpressing three heterologous genes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 50, Issue 1, 5 January 2012, Pages 5–9
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us